Evangelos Assimakopoulos studied the guitar in Athens at the National Conservatoire under Dimitris Fampas. He completed his degree in 1960 and was unanimously awarded First Prize and the Medal for Outstanding Performance. That same year he won First Prize in the Naples International Guitar Competition (Italy). Shortly afterwards he was awarded a scholarship to study with the Presti-Lagoya duo in France and Andrès Segovia in Spain.
Since 1959 Evangelos Assimakopoulos has given many solo recitals throughout Greece. In 1965 he married guitarist Liza Zoe and together they established Greece's first guitar duo. Today they are considered to be one of the leading guitar duos in the world.